Performance comparision with popular commercial analog
Immunosorbent: Monoclonal antibodies against HIV p24 Ag, HIV 1/2 antigens, immobilized on Nunc Maxisorp 96 well plate. Conjugates: Biotinilated anti-HIVp24 monoclonals, biotinilated HIV 1/2 antigens. Assay format: Two-step ELISA / sample volume 100 mkl / shaker incubation +37C / TMB substrate / total incubation time 80 min. Measurements: OD 450/620 nm
Assay: Human Immunodeficiency Virus Antigen/Antibodies combined ELISA. Poly-HRP Conjugates diluent: casein based commercial HRP stabiliser. Sample for evaluation: native HIV-1 p24 antigen (m.w. 24kDa), diluted from 20 to 0,5 pg/ml in normal human serum. Statistics: 8 repeats for each dilution point / plate dispense
Assay: Human Immunodeficiency Virus Antigen/Antibodies combined ELISA Poly-HRP Conjugates diluent: casein based commercial HRP stabiliser Sample for evaluation: native HIV-1 p24 antigen (m.w. 24kDa), diluted from 20 to 0,5 pg/ml in normal human serum Statistics: 8 repeats for each dilution point / plate dispense